Wednesday, August 31, 2011

I am in a long distance relationship and about to plan a wedding ...

I am in a long distance relationship and we are about to start planning a wedding. because we aren't able to do the typical wedding folder full of ideas I was wondering if there are any sites that provide services that specialize in this type of wedding planning. she is in Canada and I am in the U.S. the wedding will be in Canada. thanks for any help.

I am sort of in the same boat. I am in MO (where we are having the ceremony/reception) He's in TX, and his family is in MD (where we're having another reception). the best resource is family and friends. before hiring any vendor be sure to check ratings online. if you want to be involved in the planning, you guys should send a lot of emails with links/pictures of what you want. my fiance handled a lot of the online things, like buying the wedding party gifts and plane tickets. if you have any more specific needs as you get closer, let me know!

You may search for wedding planner websites which will help you in the wedding planning process.

theknot.com

the best!!!!

Tags: List of wedding ceremony participants, Long-distance relationship, ceremony reception
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Source: http://chatterbridge.info/long-distance/i-am-in-a-long-distance-relationship-and-about-to-plan-a-wedding-any-sites-that-specialize-in-this/

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Fruits, Phytochemicals & Breast Cancer | Getting Fat

If you enjoy sinking your teeth into juicy delectable fruits, you are in luck, particularly if you know a person with breast cancer or are a breast cancer individual your self. New research indicates that substances found in pomegranates and mangoes may be natural alternate options to breast cancer medication and other therapy alternatives. Pomegranates and mangoes are considered to be superfruits, or fruits that are acknowledged for their extraordinary nutritional richness and antioxidant characteristics. The fantastic issue about fruits as natural medicines is that they are scrumptious, contain a variety of vitamins and minerals, and there are no side-effects as a result of their consumption.

Pomegranates

In reference to the results of a review printed in the January issue of Cancer Prevention Analysis, researchers say theyve identified a normal compound in pomegranates that could stop the growth of hormone-dependent cancer and quit the development of estrogen-responsive tumors. Pomegranate fruit is made up of phytochemicals, specifically Urolithin B, that operate like aromatase inhibitors.

Aromatase inhibitors are employed by physicians to block the synthesis of estrogen in an endeavor to subdue the expansion of estrogen-dependent breast cancer tumors. In accordance to the Mayo Clinic web site, AI medications arrive choc-full of side effects these kinds of as severe joint ache, muscle aches, scorching flashes, headaches, bone fractures, fatigue, and even a danger of heart ailment.

The investigation crew for the aforementioned research found out that organic phytochemicals located in pomegranates suppress estrogen creation, therefore stopping the spread of cancer cells and the development of estrogen-responsive tumors. A single specific substance discovered in pomegranates ? dubbed Urolithin B ? drastically inhibited the growth of cultured cancer cells in the laboratory.

Mangos

At Texas A&M Universitys AgriLife Study division, laboratory experiments indicated that mango fruit prevented or stopped cancer progress in certain breast and colon cell lines. The experiments also showed that mango extract demonstrated some anticancer capabilities when tested on prostate, leukemia and lung cancer cells. When tested on the most typical breast and colon cancers, nevertheless, mango compounds ended up located to have even more robust cancer fighting talents than when examined on the other types of cancer cells.

In the experiments, the scientists documented that the cancer cell division procedure was interrupted by the mango extract. In truth, the mango extract triggered the colon and breast cancer cells to endure apoptosis, or programmed cell demise.

What do mangoes include that might at some point have the scientific and healthcare communities singing their praises as worthwhile adversaries of breast cancer? The solution is polyphenols, the organic phytochemicals identified in crops. Polyphenols are antioxidants with the likely to protect the physique from disease.

When husband and spouse meals experts Dr. Suzanne and Steve Talcott performed the experiments at Texas A&M, they evaluated distinct polyphenols for usefulness. What they discovered was that gallotannins were the most active cancer-fighting agents in mangoes. Gallotannins are a class of organic polyphenolic compounds thought to assist in the prevention or halt the development of cancer cells.

So, pomegranate and mango lovers, try to eat and drink up! Each fruits have the likely to alter some of people dismal breast cancer statistics by preventing the spread of cancer!

Breast Cancer Info

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Source: http://gettingfat.net/47177/fruits-phytochemicals-breast-cancer-3/

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Truth-Telling On the Campaign Trail (Powerlineblog)

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Tuesday, August 30, 2011

Meiosis: Oocytes have intracellular stream to push chromosomes off center for cell division

ScienceDaily (Aug. 28, 2011) ? Most cells rely on structural tethers to position chromosomes in preparation for cell division. Not so oocytes. Instead, a powerful intracellular stream pushes chromosomes far-off the center in preparation for the highly asymmetric cell division that completes oocyte maturation upon fertilization of the egg, report researchers at the Stowers Institute for Medical Research.

Their findings illustrate how oocytes repurposed a dynamic cellular mechanism capable of generating considerable intracellular forces and widely used by migrating cells to propel them forward, to set the stage for asymmetric cell division -- the kind of cell divisions that generate two different daughter cells. It might also lead to improvements in the selection criteria used to choose the most promising oocytes for in-vitro fertilization.

As a mammalian egg develops, it undergoes two highly asymmetric cell divisions, known as meiosis I and II. During each of these divisions, the cytoplasm divides unequally, giving rise to a large egg and two polar bodies that are much smaller than the developing oocyte. To achieve this uneven distribution of the cytoplasm, the meiotic spindle -- the structure that separates the chromosomes into daughter cells -- has to be positioned close to the so-called cortical cap, the region where the polar body will form.

"Conventional thinking predicted some sort of physical tether that moors the meiotic spindle at the cortical cap," says Rong Li, Ph.D., Stowers investigator and senior author of the study published in the August 28, 2011, advance online edition of Nature Cell Biology. "It came quite as a surprise that, instead, a continuous intracellular flow pushes the spindle into the correct position and keeps it there."

Earlier studies had ruled out microtubules, which help position the spindle during mitotic cell divisions, as potential tethers while older studies had hinted at actin as a possible candidate. Actin, one of the most abundant protein in animal cells, forms dynamic filament networks that play a crucial role in many cellular processes, including cell migration, intracellular transport.

To find out whether and how actin might play a role in the position of the meiotic spindle, postdoctoral researcher and first author Kexi Yi, Ph.D., incubated mouse oocytes with several known inhibitors of the actin cytoskeleton. "Within minutes of applying CK-666, a brand new and very specific inhibitor, the spindle drifted away from the cortical cap towards the center of the oocyte."

CK-666 inhibits the Arp2/3 complex, a major regulator of the actin cytoskeleton that is known to play a role in cell locomotion and membrane trafficking. It binds to existing actin filaments and initiates the growth of new "branch" filaments. Further experiments revealed that myosin-II contractility, better known for producing muscle contractions, pushes the spindle away from the cortical cap when the Arp2/3 complex is inhibited.

Previous work by Li and her team had shown that meiotic chromosomes, when positioned close to the cortex of an oocyte in meiosis II, induces the formation of a cortical actin cap by propagating the regulatory signal from the Ran protein. When Yi tested the effect of intercepting the Ran signal, they found that Ran also regulates Arp2/3 localization and by extension, spindle position.

Yi then turned to high-resolution time-lapse confocal microscopy and spatiotemporal correlation spectroscopy (STICS), in collaboration with Stowers imaging experts, Jay Unruh, Ph.D., and Brian Slaughter, Ph.D., both co-authors on the paper, to track the dynamics of the cytoplasmic actin network in oocytes labeled with a live F-actin probe.

"STICS analysis showed that the actin flow originates at the cortical cap and continues down along both sides of the lateral cortex before it converges near the center of the oocyte and reverses direction toward the spindle," says Yi. When he treated the oocytes with jasplakinolide, an actin filament-stabilizing drug, actin flow in the cells' interior almost immediately ceased.

"The actin flow drives cytoplasmic streaming away from the cortical cap region along the cell periphery. When it arrives at the opposite pole of the oocyte, it circulates back in a pattern similar to that of the actin flow toward the spindle," says Li. A theoretical analysis by physicist and co-author Boris Rubinstein, Ph.D., a research advisor at the Stowers Institute, found that the observed cytoplasmic streaming generates pressure on the spindle and pushes it towards the cortex.

In many vertebrate species including mammals, oocytes may arrest in meiosis II for hours or even days awaiting fertilization. "During this time the asymmetric spindle position must be stably maintained," explains Li. "Maintaining spindle position under an active force could prevent slow and random drift of spindle position or orientation if the meiotic arrest is prolonged."

Loss of asymmetric positioning of the meiosis II spindle is a known cause of impaired reproductive potential in aging females and spindle position is used as a clinical index to evaluate the quality of oocytes arrested in meiosis II for in-vitro fertilization.

Manqi Deng, Ph.D., in the Department of Obstetrics and Gynecology and Reproductive Biology in the Brigham and Women's Hospital at Harvard Medical School, Boston, MA, also contributed to the study,

The study was funded in part by the National Institutes of Health and the Stowers Institute for Medical Research.

Email or share this story:


Story Source:

The above story is reprinted (with editorial adaptations by ScienceDaily staff) from materials provided by Stowers Institute for Medical Research, via EurekAlert!, a service of AAAS.

Journal Reference:

  1. Kexi Yi, Jay R. Unruh, Manqi Deng, Brian D. Slaughter, Boris Rubinstein, Rong Li. Dynamic maintenance of asymmetric meiotic spindle position through Arp2/3-complex-driven cytoplasmic streaming in mouse oocytes. Nature Cell Biology, 2011; DOI: 10.1038/ncb2320

Note: If no author is given, the source is cited instead.

Disclaimer: Views expressed in this article do not necessarily reflect those of ScienceDaily or its staff.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/QDgL-lek7AE/110828140945.htm

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Q & A: Any advice on accounting ... - Small Business plan software

Q & A: Any advice on accounting / bookeeping two employees of a contractor?

Josielynn22 issue ? Any advice on accounting / bookeeping contractor for two people
My husband and I have our own team of a brick paving, we have only two employees, and I have to figure out how to go about doing payroll ??. Can someone give me a program and / or system that includes these things * Salary * Employee representative tax bases, and white * withioldings AufwendungenIch income and protocols, this will help a lot of different software and online services, but I hope someone with similar business situation, which has some knowledge of what works best, the best answer to listen .

response to Jeffrey K
Yes, I would only recommend the program for its simplicity and flexibility. QuickBooks is the best overall program out there.


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Irene moving up mid-Atlantic states; moving NNE (AP)

MIAMI ? A weakened but still dangerous Hurricane Irene shut down New York and menaced other cities more accustomed to snowstorms than tropical storms as it steamed up the East Coast on Saturday, unloading a foot of rain on North Carolina and Virginia and knocking out power to 2 million homes and businesses. At least eight people were killed.

New York emptied its streets and subways and waited with an eerie quiet. Washington braced for the onslaught, too, as did Philadelphia, the New Jersey shore and the Boston metropolitan area. Packing wind gusts of 115 mph, the hurricane had an enormous wingspan ? 500 miles ? and threatened a swath of the nation inhabited by 65 million people.

The hurricane stirred up seven-foot waves. At New York Harbor, even with the eye of the storm several hours away, there was a 3.5-foot storm surge early Sunday.

Across the Northeast, drenched by rain this summer, the ground is already saturated, raising the risk of flooding as well as the danger of trees falling onto homes and power lines.

Irene made its official landfall just after first light near Cape Lookout, N.C., at the southern end of the Outer Banks, the ribbon of land that bows out into the Atlantic Ocean. While it was too early to assess the full extent of damage, shorefront hotels and houses were lashed with waves, two piers were destroyed and at least one hospital was forced to run on generator power.

"Things are banging against the house," Leon Reasor said as he rode out the storm in the town of Buxton, N.C. "I just hate hurricanes."

Eastern North Carolina got 10 to 14 inches of rain, according to the National Weather Service. Virginia's Hampton Roads area was drenched with at least nine inches, with 16 reported in some spots.

By late Saturday night, the storm had sustained winds of 80 mph, down from 100 mph on Friday. That made it a Category 1, the least threatening on a 1-to-5 scale, and barely stronger than a tropical storm.

Nevertheless, it was still considered highly dangerous, capable of causing ruinous flooding across much of the East Coast with a combination of storm surge, high tides and 6 to 12 inches of rain.

There were no hurricane-force winds over land Sunday morning; they were limited to a relatively small area over the Atlantic Ocean as the eye of the storm traveled over water after touching down in North Carolina. But tropical-storm-force winds still covered a wide area of land, and Irene was still expected to be a hurricane when its eye made landfall again in the New York area around noon Sunday.

Irene was moving north-northeast at 17 mph, slightly faster than it had been Saturday, and was centered about 195 miles south-southwest of New York City. A typical hurricane would be moving much faster, 25 to 30 mph, said senior hurricane specialist Stacy Stewart of the National Hurricane Center.

Pennsylvania Gov. Tom Corbett warned that the state will not necessarily be out of danger once the storm has passed: "The rivers may not crest until Tuesday or Wednesday. This isn't just a 24-hour event."

As of Saturday evening, Irene was hugging the U.S. coastline on a path that could scrape every state along the Eastern Seaboard. Ed Rappaport, deputy director of the National Hurricane Center in Florida, said it would be a "low-end hurricane, high-end tropical storm" by the time it crossed the New York City area late Sunday morning.

The storm is so large that areas far from Irene's center are going to be feeling strong winds and getting large amounts of rain, he said.

"It is a big, windy, rainy event," he said.

The deaths blamed on Irene included two children, an 11-year-old boy in Virginia killed when a tree crashed through his roof and a North Carolina child who died in a crash at an intersection where traffic lights were out. Four other people were killed by falling trees or tree limbs ? two in separate Virginia incidents, one in North Carolina and one in Maryland. A surfer and another beachgoer in Florida were killed in heavy waves.

Power outages were concentrated in Virginia and North Carolina.

Irene was the first hurricane to make landfall in the continental United States since 2008, and came almost six years to the day after Katrina ravaged New Orleans on Aug. 29, 2005. Experts guessed that no other hurricane in American history had threatened as many people.

North Carolina Gov. Beverly Perdue said Irene inflicted significant damage along her state's coast, but that some areas were unreachable because of high water or downed power lines. "Folks are cut off in parts of North Carolina, and obviously we're not going to get anybody to do an assessment until it's safe," she said.

At least 2.3 million people were under orders to move to somewhere safer, though it was unclear how many obeyed or, in some cases, how they could.

Defense Secretary Leon Panetta told 6,500 troops from all branches of the military to get ready to pitch in on relief work, and President Barack Obama visited the Federal Emergency Management Agency's command center in Washington and offered moral support.

"It's going to be a long 72 hours," he said, "and obviously a lot of families are going to be affected."

In New York, authorities undertook the herculean job of bringing the city to a halt. The subway began shutting down at noon, the first time the system was closed because of a natural disaster.

On Wall Street, sandbags were placed around subway grates near the East River because of fear of flooding. Tarps were spread over other grates. Construction stopped throughout the city, and workers at the site of the World Trade Center dismantled a crane and secured equipment.

The city was far quieter than on an average Saturday. In some of the busiest parts of Manhattan, it was possible to cross a major avenue without looking, and the waters of New York Harbor, which might normally be churning from boat traffic, were quiet. About 370,000 people living in low-lying areas of the city, mostly in Lower Manhattan, Brooklyn and Queens, were under orders to clear out.

"The time to leave is right now," Mayor Michael Bloomberg said at an outdoor news conference at Coney Island, his shirt soaked from rain.

The New York area's major airports ? LaGuardia, Kennedy and Newark ? waved in their last arriving flights around noon. The Giants and Jets postponed their preseason NFL game, the Mets postponed two baseball games, and Broadway theaters were dark.

New York has seen only a few hurricanes in the past 200 years. The Northeast is much more used to snowstorms ? including the blizzard last December, when Bloomberg was criticized for a slow response.

Airlines said 9,000 flights were canceled, including 3,000 on Saturday. The number of passengers affected could easily be millions because so many flights make connections on the East Coast.

Greyhound suspended bus service between Richmond, Va., and Boston. Amtrak canceled trains in the Northeast for Sunday.

In Philadelphia, Mayor Michael Nutter declared a state of emergency, the first for the city since 1986, when racial tensions were running high. "We are trying to save lives and don't have time for silliness," he said.

The storm arrived in Washington just days after an earthquake damaged some of the capital's most famous structures, including the Washington Monument. Irene could test Washington's ability to protect its national treasures and its poor.

In New Jersey, the Oyster Creek nuclear plant, just a few miles from the coast, shut down as a precaution as Irene closed in. And Boston's transit authority said all bus, subway and commuter rail service would be suspended all day Sunday.

___

Mitch Weiss reported from Nags Head, N.C. Associated Press writers contributing to this report were Tim Reynolds and Christine Armario in Miami; Bruce Shipkowski in Surf City, N.J.; Geoff Mulvihill in Trenton, N.J.; Wayne Parry in Atlantic City, N.J.; Eric Tucker in Washington; Martha Waggoner and Gary D. Robertson in Raleigh, N.C.; Jessica Gresko in Ocean City, Md.; Mitch Weiss in Nags Head, N.C.; Alex Dominguez in Baltimore; Brock Vergakis in Virginia Beach, Va.; Samantha Bomkamp and Jonathan Fahey in New York; and Seth Borenstein in Washington.

Source: http://us.rd.yahoo.com/dailynews/rss/weather/*http%3A//news.yahoo.com/s/ap/20110828/ap_on_re_us/us_irene

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Monday, August 29, 2011

Gary Berg's Trip to Europe Demonstrates Again ? Entertainment is ...

Gary Berg, Founder of G. L. Berg Entertainment, recently?traveled to Holland, Belgium, and France.? Among the many exciting experiences he enjoyed, seeing street performers, musicians in the park, European comedy clubs, and city festivals happening everywhere reminded him how?much we share as?human beings and how basic principles and activities are similar across cultures.? What often makes traveling special is participation in the arts, cultural, and entertainment events that a particular region has to offer.?

Everyone has music.? Everyone has laughter.? Everyone has art, in many forms.? The beauty of traveling is to see how each culture puts its own stamp on arts and entertainment?to create its own twist on those experiences.? Whether it is circus arts, folk music, stand-up comedy, magic, or juggling, it all has similar roots, but each culture defines the way it is presented and created.? You see this in artist?s costumes, use of scenery, instrument and prop choices, and more.? We come home with new experiences, a new way of looking at life, and memories that enrich our lives.

In Minnesota, we are fortunate?to have?dedicated funding that supports?investment in arts, history, and cultural heritage programs.? In 2008, Minnesota voters passed a constitutional amendment creating a 3/8 cent sales tax to support outdoor heritage, clean waters, sustainable drinking water, parks and trails, arts, history, and cultural heritage projects and activities?with a goal of ??providing every Minnesotan lifelong access to programs and activities that engage him or her as innovator, historian, entrpreneur, and creator of Minnesota?s future.??? The innovation initiated through Minnesota?s Legacy Amendment?hopes to ?bolster Minnesota?s reputation as a center for creativity, innovation and imagination, supporting the long-term vitality of our state.?

Gary?s trip showed him once again that entertainment is universal and creates a better?place to?live.? The things we like about vacation often also make our communities a better place to live, and that is why Gary and the G. L. Berg staff is so committed to bringing live entertainment and performing artists to Minnesota, the Upper Midwest, and the country.? As Gary recently commented:? ?Work is work and stuff is just stuff but people and entertainment,when combined, are special and memorable.?? We hope you will be able to enjoy G. L. Berg performing artists and entertainers often as you participate in fairs, festivals, city parks, resorts, theaters, and private events throughout the upcoming months.

Theatre Du Vaudeville, Brussels, Belgium

Eiffel Tower, Paris, France

Art is Everywhere in Amsterdam - Canal in Amsterdam

Source: http://www.glberg.com/2011/08/gary-bergs-trip-to-europe-demonstrates-again-entertainment-is-universal/

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My City Mommy ? Business Week Year Subscription $12.99 (Retail ...

Posted by My City Moms on Sunday, August 28th, 2011 at 7:48 am

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This is a great deal for yourself or to purchase as a gift, so head over and grab this one while you can!

Tags: Daily Deals

Source: http://www.mycitymommy.com/2011/08/business-week-year-subscription-12-99-retail-249.html

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Farmers Branch Property Management was Simple with real estate ...

August 27, 2011 at 10:04 pm?-?Views: 9

To become owner of a family-owned apartment simple or complex, it can be quickly overwhelmed with the tasks of management of property of Farmers Branch that must be undertaken. Be in compliance with all State and federal regulations to respond to all calls-keeping tenant, can be a stressful experience especially when they?re new to the scene of the investment. This is where the administration of property estate can come in and help make this a simple and stress free process for you and your tenants. There are many owners who decide to manage their real estate in Farmers Branch on their own. While this is a good idea for some is that it is overwhelming to find, others take care of all the details when they start to grow their portfolio. When are you new to the investment scene can be quite stressful learning the rules and stay up to date while still working your full time job. A landlord has become a full time job as the tenants on you for all their needs confidence when problems arise. You must be prepared for a situation that your way if you choose to do this only. Some find they enjoy it and put this in their full time job. Others hire Farmers Branch property managers to help them and enjoy the free time, that it gives them. One of the advantages of hiring farmers branch property manager to help with your car rental needs, so that you get your time back. No more midnight phone calls or hunting down reliable contractors to do the job. You can let our team of service 24 hours a day, to handle these calls for you. We have the staff and assist with any repair necessary list of preferred suppliers. By doing this, we will help you to minimize the cost of having the properties maintained. Our tenants as the security that they get because they know that no matter what time, if there is a problem we will be there to help them. Take a look around our site management of real estate and see what we can offer the user a bit. We can help provide protection for themselves and their own resources and help you manage the properties of the Branch to farmers. This means, don?t worry if you are in compliance, because you will know that you are.

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Sunday, August 28, 2011

Once in a Lifetime Fly Fishing on the Cimarron River | Texas real ...

Two of my favorite New Mexico rivers to fish are the San Juan River and Cimarron River. This story is about the Cimarron. The Cimarron River is in Northeast New Mexico and emanates out of Eagle Nest Lake. Located on the eastern edge of the Sangre de Cristo Mountains, this dry fly gem flows eastward through Cimarron Canyon State Park along US Hwy 64. After flowing into several other rivers this water ultimately reaches the Mississippi River. New Mexico Game and Fish estimates nearly 4,000 catchable sized wild browns per mile, this is unquestionably the healthiest wild brown trout fishery in the state and one of the healthiest in the southern Rocky Mountains. Most of the wild browns are 10-14 inches with the occasional big boy. This fishery is not well known but can still get a little crowded close to the roads during mid summer.

I can easily get to the Cimarron within a one-day hard drive from my Fly Shop near Tyler, Texas and be fishing late that afternoon or early the next morning. The high mountain scenery is beautiful and the fishing is great.

My story begins in the middle of June 2002. During June, the stonefly hatch is in full swing on the Cimarron and big browns will eagerly attack a size 14 or 16 Stimulator with regularity. This is my favorite time of year to fish this river. This is dry fly fishing at its best.

Unfortunately, if you remember, this was during the time wild fires were engulfing many parts of New Mexico, Colorado and Arizona. The Cimarron Canyon State Park was surrounded by a couple of such fires.

I arrived at the Pine Ridge Hotel, only about a mile from quality fishing water, late Monday evening. This is a quaint little four-room hotel nestled near the entrance of the state park. After checking in, the hotel owner told me that the park was closed and had been since Friday. Because of the fire dangers, it was closed to everything including camping, hiking, picnicking and YES? fishing. Every park pull off was barricaded in bright yellow. It seems like every other tree had a Park Closed sign nailed to it. I couldn?t believe it. I had driven 12 hours strait to spend some personal time dry fly fishing and? wouldn?t you know it? No Fishing. I was sick. If you have ever wanted to see a big man cry this would have been a great opportunity.

There were no other places near by that I could fish unless I had a guide to fish some private waters that were near by. I called my good friend, Doc Thompson, of High Country Anglers. Doc is the best fly fishing guide for the small streams of Northeast New Mexico including some private areas of the Cimarron. He was booked. No chance for me to fish any private water.

I was considering cutting my losses, packing up my gear and heading home with my tail tucked between my legs when I saw the park ranger turn into the motel parking lot. I eagerly approached him, explained my predicament and pleaded with him to be merciful on this misguided Texan. He indicated that there was little he could do. He was ordered by higher-ups to close the park. The area was very dry and the fear of the forest fire spreading was at an all time high. He finally gave me a miniscule window of hope when he said that if the wind didn?t pick up or change directions during the night he would ask if he could open the park the next day. No guarantees. There was only a small chance of things falling my way but I decided to wait it out and see.

The next morning, bight and early, I walked to the hotel owners desk with my fingers and toes crossed. ?Great news? the owner said, ?you can fish.? The park ranger called the hotel and told the owner to go tell Tex he can fish. The ranger opened the park to fishing only. No one could camp, hike or picnic. Fishing only. Best of all, he left all of the barricades and Park Closed signs up. My head began to buzz with this scenario. Could it be true? Is it possible that any where in this country there is a stream that is full of big browns, full of fish eager to slam dry flies, has had no fishing for five days, has Park Closed signs on every other tree and has bright yellow barricades at every pull off? Is this really happening? And most of all? is this really happening to me? YES? it was!!!

I almost broke a leg and a fly rod getting to river so quick. I was the only person fishing this 12-mile stretch of quality trout water. The ONLY person. The river has had no fishing pressure for five days. None. Notta. Absolutely zero. What a magnificent opportunity. I hit the water at 8:00 am. Knowing that the stonefly hatch would began at a bout 9:00 am, I tied on a size 16 yellow Stimulator with a Copper John dropper on my 2wt rod. For the next hour, the catch was 50/50 on the dry and nymph. When the hatch started, I removed the nymph in order concentrate on the dry. My stimulator was repeatedly demolished by hungry trout. The hatch started around 9:00 am and eventually tapered off around 2:00 pm as the day warmed. During that time, I landed more than 40 nice brown on a dry. Most of them were in the 10 to 12 inch range. Some a little smaller and some a little bigger. The whole time, I saw no other person on the river. I was in total fly fishing bliss. Later that afternoon, as the temperature began to drop, they started hitting hard again and did so until just before dark. I lost count of the total number of fish that I caught that day. After a while you just quit counting. The fishing was phenomenal and the solitude was even better.

During the day, when I would take brake at my parked Jeep for a shot of Joe or something, people would drive buy giving me the stare of disgust. I could see their lips mumble something like, ?that idiot Texan? can?t he read the signs?? I was in no way interested in setting them strait. I wanted the entire state on New Mexico as well as the whole wide world to think the park was completely closed and may never open again.

That evening, back at my room, the harsh realities of that summer were vivid. I sat on my front porch and watched the helicopters and other large aircraft drop loads of water on the forest fires that were scattered along the mountainside. Huge walls of smoke draped the horizon. I couldn?t help but become sorrowed because of the destruction that was unfolding before my eyes. When it was too dark to see, I retired to the confines of my room.

Later that night, as I lay packed tight in my cozy little bed, I found it difficult to sleep. As my mind drifted from the fires outside and danced with thoughts of my day of fishing, I giggled as I felt myself swelling with contentment. My angling experience kept running through my mind. I couldn?t help but wonder what the next day would bring. Would my fly fishing solitude come to an end? Guess what? it didn?t.

I hit the park early the next morning to be faced with the very same scenario. The signs and barricades were still in place and no one was in sight. Using the same flies and techniques I enjoyed equal success as I had the day before. It wasn?t until late that afternoon did I see the only other car in the park. The word was finally out. By then, it was OK. I enjoyed a few hours of good fishing the next morning before heading back to Tyler.

Is there a moral here? I don?t know. It was defiantly a fishing trip that I will never forget. In retrospect, however, it seems really unfortunate that my memorable fishing trip was at the expense of so much. The southwest lost an awful lot of good forest and helpless wildlife during that horrible time. Homes were gone and lively hoods were destroyed. I suppose the saying remains true, ?someone?s good fortune is usually at someone else?s expense.?

By: Steve Robbins

Source: http://www.greenchristmasappeal.org/recreation-and-sports/once-in-a-lifetime-fly-fishing-on-the-cimarron-river

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playGo USB does wireless audio slightly cheaper than a Sonos

Audiophiles wanting to wirelessly liberate their music from desktop speakers have a tough choice -- buy a $99 Airport Express, which uses a less-than-ideal 3.5mm line-out jack, or spend big on a Sonos. Bicom's playGo USB is the new cord cutting kid on the block, and it's aiming for the middle slice of that market. For $450 you get a transmission unit which connects via microUSB to your computer and a receiver that connects to your HiFi with RCA or Optical cables. Song streaming couldn't be easier, the two have a range of 75 feet and pair up automatically over the 2.4 GHz band to send 24-bit sound to your beloved home audio kit. To stand out from the crowd, the playGo is hewn from Corian (yes, the stuff used for kitchen counter tops) and has a layer of LEDs to add some color to your musical experience. It's currently on sale for $400, but let's be honest -- you can't put a price on freedom from wires, can you?

Update: The Airport Express' line-out port is a hybrid -- capable of using analog or optical cables.

[Thanks to everyone who sent this in]

playGo USB does wireless audio slightly cheaper than a Sonos originally appeared on Engadget on Thu, 25 Aug 2011 21:38:00 EDT. Please see our terms for use of feeds.

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Source: http://www.engadget.com/2011/08/25/playgo-usb-does-wireless-audio-slightly-cheaper-than-a-sonos/

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NovaJet Private Jet Charter - IAD Mag Travel Site

Charter a Private Jet with NovaJet and you step into a world of unparalleled service and personalized attention to detail. With their private air charter concierge service, no request is too great or too small. It’s what distinguishes them as Toronto’s preferred Executive Jet Charter choice. And it means you can simply sit back and enjoy a perfect Charter flight.

Source: http://www.iadmag.com/travel-and-leisure/novajet-private-jet-charter/

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Salt Lake City Food - Food & Drink: Wine & Cheese Benefit Party

Posted // August 26,2011 -

Crossroads Fundraiser
The Crossroads Urban Center, which works to help the underserved by providing emergency food, diapers, infant formula and clothing to those in need, will host its ninth-annual Wine & Cheese Benefit Party at The Bayou on Sunday, Sept. 18, from 1-4 p.m. Wine, nonalcoholic beverages, gourmet cheeses, assorted breads and fresh fruit will be served, and the local band Controlled Burn will perform. Tickets cost $45 per person, and all proceeds benefit Crossroads Urban Center. ?Crossroads works to address the root causes of poverty and injustice, not merely the symptoms. This means we must make an unwavering commitment to organizing the community and involving people in the decisions that affect their lives and impact their ability to be self-sufficient,? says Glenn Bailey, the center?s executive director. ?The Wine & Cheese Party is a great chance to have fun and support valuable community services for those in need.? In addition to The Bayou, Tony Caputo?s Market & Deli, Kiler Grove Winegrowers and Harmons are donating food and wine for the event. To buy tickets in advance, phone 801-364-7765.

Fleming?s Winesdays
Aside from very good steaks, seafood and other tempting menu selections?not to mention top-notch table and bar service?one of the things that keeps me coming back to Fleming?s Prime Steakhouse & Wine Bar is the Fleming?s 100. That refers to the 100 wines that Fleming?s offers by the glass?many more than any other local restaurant I?m aware of?ranging from sparkling wines and interesting whites from around the world, to big, bold reds from producers such as Orin Swift, Raymond, Trefethen, Rodney Strong, Jean-Luc Colombo and many more. And as though that weren?t enticing enough for wine lovers, Fleming?s is now offering complimentary corkage on your own bottle of wine every Wednesday during ?Winesdays? in September. By the way, here?s something you might not expect: Complimentary corkage is not allowed in 13 states, including Arizona, Colorado and Massachusetts, but it is here. So, order up a delicious Prime steak, and uncork one of those special bottles you?ve been saving.

Quote of the week: Hunger is not debatable. ?Harry Hopkins

Ted Scheffler Twitter: @Critic1

Source: http://www.cityweekly.net/utah/article-297-14575-wine-cheese-benefit-party.html

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Saturday, August 27, 2011

Articles 20VN | Cruising Online on a Budget | Best deals | Online ...

[unable to retrieve full-text content]Now that you are past this (largely) mental hurdle you can make an informed comparison at your own leisure and time and click on to the link of the best deals online. You would first have to decide on your destination. Is it Hawaii or Havana, Jericho or Jakarta? ... Cruise lines like the Royal Caribbean and Carnival come with wide berths and adjoining commodious cabins, even family suites. Also, do not forget to enquire about the age limit pertaining to child travel. ...

Source: http://www.20vn.com/shopping-product-reviews/cruising-online-on-a-budget-15d.htm

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Business Process Outsourcing Evolving To The Cloud Contact ...

Saturday, August 27th, 2011 at 7:25 am ?

Web solutions and interactive platforms are advancing to the next level every day. With new apps, websites, and mobile devices on the market daily it?s easy for a consumer to do just about anything. Companies are having an easier time of it as well, technology helps them manage projects, finances, and more. The new technology for business process outsourcing is The cloud contact center.

Gain a better understanding of a cloud contact center.

The transformation is happening. An easier, more versatile, cost effective, web based platform for business process outsourcing is making its move. The cloud contact center is leading the way in web based platforms for corporations to implement and conduct business process outsourcing. Results have been tremendous for those businesses that have already converted to the cloud contact center. From financial to customer care improvements, business process outsourcing is becoming far more efficient and cost effective than even just a few years ago.

Learn how business process outsourcing can make your business more efficient.

Why all the hype around the cloud contact center? Using the cloud contact center is changing the nature of business process outsourcing for both the internal user and the external customer. From an internal point of view, most call centers have a large facility, after converting to a cloud contact center web based call center an employee can work from home, anywhere. With customers able to shift work to the home office there?s no need for a big facility, takeout the facility and a huge expense is lifted. In addition to losing the real estate, without a large facility there isn?t a need for a full IT staff. By using the cloud, and cutting these two huge expenses some companies have seen costs cut up to 50 percent.

When using the cloud contact center customer relations will become stronger. With the cloud in place, information will be readily available making it easier for employees and customers to communicate. With improved customer relations, businesses will see higher customer return rates and then higher revenues.

Does your company need a cloud contact center?

In the near future business process outsourcing will be fast, efficient and relatively inexpensive. Using the cloud contact center is cutting edge and will help a business progress in the future with relative ease.

Tagged with: business process outsourcing ? Cloud Contact Center

Filed under: Uncategorized

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Source: http://hmjproject.com/business-process-outsourcing-evolving-to-the-cloud-contact-center/

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Would a Palin 2012 run make her 'disappear'? (The Week)

New York ? Sarah Palin keeps stealing the 2012 spotlight, and she is not even a candidate. What happens if she jumps into the race?

The Palin prognostications continue. Sarah Palin's political action committee ? reacting to fresh Palin-for-president speculation from Karl Rove ? said this week that anyone who claims to know her plans is trying to "mislead the American public." Meanwhile, Salon's Steve Kornacki opined that anyone tired of seeing?the former Alaska governor consume "all of the media oxygen" should hope she does jump into the race. The likely result, says Kornacki: An "epic, humiliating defeat" that would make her "disappear" politically. Would a Palin run really be her undoing?

Sorry, Palin haters, that is wishful thinking: While a Palin run seems fairly unlikely, "I am not sure it is possible for Palin to alienate her core audience"?even if she lost, says Steven L. Taylor at Outside the Beltway. In fact, I think a run could still help her maintain her "medium level politico-celebrity level well into the future." After all, "since a lot of her shtick is based on being the victim of ye olde lamestream media, defeat has a certain cache."
"Would running spell the end of Palin?"

Not running would make her irrelevant: Actually, Kornacki might have a point, says John Sides at The Monkey Cage. "But here's another way to make Palin disappear: have a presidential election in which she is not a candidate." Any prominent Republican who's not in the race will have to sit idly on the sidelines while "the candidates, and then the ultimate nominee, will suck up virtually all of the oxygen."
"Making Palin 'disappear'"

Palin is not going to disappear, period: Kornacki's theory that Palin would end up in obscurity, like the Gary Hart did in 1988, is amusing, says Jonathan Bernstein at A Plain Blog About Politics, but even a loss wouldn't make her a "joke" like the Democrat Hart was. Hart was left with nothing when, humiliated by scandal, he ceased to be a viable candidate. Conservatives who are "very hostile to the GOP 'establishment'" will stick with Palin, win or lose.
"How to make Palin go away"

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Source: http://us.rd.yahoo.com/dailynews/rss/politicsopinion/*http%3A//news.yahoo.com/s/theweek/20110825/cm_theweek/218529

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Friday, August 26, 2011

History of running track

Athletics is one of the most popular events of the Olympic Games. Each year there are old records and new narratives emerge in the sport exciting and evolving. What is the history of career? It is a long history to look back, the athletics competitions in ancient Greece.

An old sport

The execution took place in ancient Greece, where race was going, the first and only event at the Olympic Games, the original. In addition to the Olympic Games,Pan-Hellenic games allowed the runners to compete at regular intervals. The Roman games, including the execution of the same era, but more focused on fighting and fighting. As far away as Ireland, ancient Europeans competed in races in the games Tailteann. The ability to run was considered an essential talent in the ancient world, especially for warriors.

The Middle Ages

Sports will continue to track performance throughout the Middle Ages. In Europe, the noble families in their children's educationa variety of athletic skills to improve their combat skills. The execution was just one of many sports that were shown in competition at this time. However, the increased use of the horse infantry instead of the war has taken the focus away from personal rate, rather than draw attention to the skills of horsemanship and weapons.

The Olympic Games Revived

In the late eighteenth century, was the long and glorious history of athletics and other sports field and recognized alongwith the need to revive the traditional Greek games in some form. France L'Olympiade de la R?publique characterized by a series of ancient sport, with the management of the foot a prominent place in the competition. The metrics used for the first time in those games to measure and record the time, another milestone in the history of the race.

The execution will become more popular during the nineteenth century as a physical form is gradually recognized as a key to health and vigor. Schools began, includingto compete with physical activity and competitive sports in their classes, a generation of young athletes in athletics.

The modern sport

The performance was one of the original sports in the 1896 Olympic Games. Although women were excluded until 1928, soon took a leading position in sports. The sport has developed his current car, with most activities on an oval track 400 meters. A person, with the route in this day and age canchoose from any number of events including sprints, hurdles, middle and long distance races and relays. Each event has a unique set of ideal characteristics, the production of a career in the sport more diverse and inclusive world.

Although the history of athletics is long and includes contributions from a variety of cultures is not complete. While the new emerging athletes to improve with each generation and new technologies, the sport's historywritten every time I pass runners in the field.

Source: http://recreation-track-and-field.chailit.com/history-of-running-track.html

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Alaska oil field security guard kills polar bear (Providence Journal)

Share With Friends: Share on FacebookTweet ThisPost to Google-BuzzSend on GmailPost to Linked-InSubscribe to This Feed | Rss To Twitter | Politics - Top Stories Stories, News Feeds and News via Feedzilla.

Source: http://news.feedzilla.com/en_us/stories/politics/top-stories/127426111?client_source=feed&format=rss

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How Kids And Teens Differ? at CarpetSweeperPlus Blog

Kids? life and teens? life are in two different demographics. The time between being a child and full adult is called Teenage time. Teenage is usually considered to begin around age 9, and end around 18. Not only on the basis of intelligence and book smarts but also in the name of experience both teens and kids differ. Many kids want to change their friends and like to be in a different group. Majority of kids say they like to read books for fun and that reading books for fun is important and exists a correlation between reading and success.

Teens know what?s best for kids and kids know what?s best for them. Teens have experience and kids want experience. Adult time is different from kid time. One is slower than the other; one takes longer than the other. Teens want to talk and kids want to act. The tension isn?t between talk and action, because both are action. The tension between kids and teens over who is or should be in charge is related to issue of power, control, experience and personal development.

As teens develop both physically and emotionally and as their awareness of self changes, it can be tempting for teens to compare themselves with others as part of the natural desire to feel accepted by their peers. The brain undergoes many changes during the teen?s years. Teens know what?s best for kids and kids know what?s best for them. Teens have experience and kids want experience.

Teens want to take some time and think it over, and kids want to do it yesterday. Teens want to talk and kids want to act. Teens think about consequences and kids think about now. Teens think they have to be in charge, and kids want a chance to be in charge. Teens can ?hang in there? longer than kids, who get bored easily and quickly and want a change of task or job. Teens think that it will be too expensive and that kids don?t understand it. Teens really don?t respect kids, but expect kids to respect them.

They won?t be teenagers forever, only adults whose brains are rewiring in decision making, impulse control and judgment making connects all of them. Certainly this could account for much of the irrational decisions and mood swings. The world depends on that. The world depends on us!

Patricia is an avid professor and pushes for teaching profession in the Washington. She has a team of Virtual Personal Assistants who help her run her site and her website as well as manage many blogs that she runs. If you?re interested in Patricia?s coaching world or you would like to find out more on how her Virtual Personal Assistant works, visit her website. Virtual Personal Assistant

Source: http://carpetsweeperplus.com/blog/2011/08/how-kids-and-teens-differ/

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Thursday, August 25, 2011

All Businesses Need A Social Media Consultants To Grow Your Profit ...

Times have changed for the average business that it has become more and more dificult to market products or services, and many business owners are turning to the more updated tactics to find potential customers. Social Media Marketing is becoming more and more untilised as the results are astounding. All the work that the owner of a business social media consultant to do, will still be present in the coming years that an ad in the local newspaper will be there for a week or two and then disappeared for good.

All the major brands have facebook pages, Twitter accounts, blogs, video and many articles are working to find new customers or clients. More customers clients mean more money. The primary goal is to provide a professional and expert, able to give advice when needed and easily accessible via the Internet.

It has been proven that the mobile phone is the main way people use the Internet to find the information they need whether it?s a restaurant or a plumber, even the home computer. So this only says one thing, yellow pages and local print ads are out and marketing on the Internet in any way possible is in.

]]>

The question is, how a business promote themselves online? The best way is to first ensure that the main site is optimized for Internet so that search engines now what the site is about. As company will eventually go out and spread the word about what the company is and what they sell, whether it is a service or product. The problem with this is that the entrepreneur will not have time to do this or they are not knowledgeable enough to do the job. This is where a social media consultant arrives

A social media consultant will go over all aspects of a company and create a marketing plan that fits the business in hand. From the SEO aspects of the most important area to put the business on google map and then on to the social media platforms. The choice of how much campaign should be identified and subsequently implemented in an expeditious manner.

A continued maintenance of social media platforms would be a good idea that the business owner may not have time to be busy adding content on a regular basis to keep everything fresh and new.

A good social media consultant could mean that a company could grow at a much faster pace. Add videos and articles to a growing company might have great success if done correctly and that means the company is in any type of media being sought in the search engines. Talk simultaneously with the public on their own turf, will make potential customers clients more relaxed and will see the company as an expert in the field.

Social media consultants are a must for any serious entrepreneur who wants their business to grow and be known all over the internet as the experts. Social media marketing is the right way to go if advertising a business is necessary. Learn from the big companies that use this type of marketing to make money. They can not all be wrong.

Related Social Media Consultant Articles

Related Posts:

Tags: Business Owners, Businesses, Consultants, Entrepreneur, Expert Advice, Google, Grow, Home Computer, Job, Major Brands, Margin, Marketing On The Internet, Marketing Plan, Media, Media Consultant, Media Consultants, Media Marketing, Media Platforms, Mobile Phone, Need, Plumber, Print Ads, Profit, Profit Margin, Search Engines, Social, Social Marketing, Yellow Pages

Source: http://www.onlinesocialmarketingtips.com/social-media-manager/all-businesses-need-a-social-media-consultants-to-grow-your-profit-margin/

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Find Success by Looking At Your Personal Problems - ArticleMS

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by: 01stefany.gale82
Total views: 0
Word Count: 581
Date: Thu, 25 Aug 2011 Time: 9:28 AM

People come to the Internet every day hoping to make money, and never even consider that their success depends on self improvement. Since most people who come online trying to make money fail, why is it so hard to believe that it is because of lack of self improvement? What we are talking about with self improvement is whatever is going on inside people that prevents their success. All people have a way of thinking that leads them either on the path of success, or on the path of failure, so the right thinking is imperative to success. Attitudes were put in, and they can be taken out, but no one is saying that it is something that will be easy. When you decide to build an online business, learning to remove the mental blocks holding you back from success, will be the hardest thing you need to do. Without the right self improvement, your business will never become a thriving achievement.

You will witness people slowly gaining comfort with expression as you pay attention to those that work in online business. After a time, advice for things like differing fears, procrastination and all kinds of issues will be sought by people. Those are the single greatest obstacles to realizing the dreams of business success. The private struggles are the largest hindrances because as you battle with the subconscious, your conscious mind will be the loser. To see what is actually happening, begin to sort out your own puzzles to find knowledge of your consciousness and memories.

Using this strategy, you will be able to improve your confidence and business skills as you move closer toward financial independence. The initial strategy that you must implement is never thinking about your lack of confidence in any given situation.

Simply accept that it is there, and give it as little energy in your mind as possible. Then, focus on one thing you need to do for your business and work on that until it is complete. Your goal, no matter how long it takes you, is to complete this one task. Your next step is to do one more job or task until it is complete. Doing this will keep your mind focused on what you need to complete without spinning out of control and forgetting about what you need to do.

Have you ever paid attention to your thoughts over a period of time? If you have, do you feel the way you think is negative or positive? If thoughts lean to the negative for the most part, then you will be able to use that ascertainment to make your chances better of acquiring what it is that you desire in your personal life and occupation. Many people do not realize that negative thought process reveals much. If you think in that manner, it doesn't just happen without cause. For example, if you frequently think something will not work, such as a business idea, then that is only the manifestation of deeper beliefs. What you believe in the deepest part of you is what needs to be looked at if you want things to change.

Once you understand the problem, seek professional help, or try to fix the issue yourself. You have within you the power to overcome anything so always keep that in mind as you move toward success. Belief in what is possible is powerful and will see you through.

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DataLister Workers Compensation Prospecting | Best Finances

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Source: http://bestfinancesolutions.com/insurance/credit-insurance/datalister-workers-compensation-prospecting/

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Christopher Knight Files For Divorce From Adrianne Curry

Christopher Knight Files For Divorce From Adrianne Curry

The former “Brady Bunch” star Christopher Knight has filed for divorce from his reality television star wife, Adrianne Curry. This news sucks…we had hoped the [...]

Christopher Knight Files For Divorce From Adrianne Curry Stupid Celebrities Gossip Stupid Celebrities Gossip News


Source: http://feedproxy.google.com/~r/stupidcelebrities/~3/eT9bmAVa4pk/

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Wednesday, August 24, 2011

TGen and DTU researchers track source of Haitian cholera outbreak

TGen and DTU researchers track source of Haitian cholera outbreak [ Back to EurekAlert! ] Public release date: 23-Aug-2011
[ | E-mail | Share Share ]

Contact: Steve Yozwiak
syozwiak@tgen.org
602-343-8704
The Translational Genomics Research Institute

Lessons from 2001 anthrax case help pinpoint source of disease

FLAGSTAFF, Ariz. Aug. 23, 2011 Employing technology that reads the entire DNA code, researchers led by the Translational Genomics Research Institute (TGen) and the Technical University of Denmark (DTU) have pinpointed the source of a cholera outbreak in Haiti that killed more than 6,000 people and sickened 300,000.

Using whole genome sequencing, which spells out the billions of chemical bases in DNA, TGen and DTU provided the strongest evidence yet that peacekeepers from Nepal, where cholera is widespread, brought the disease to Haiti, which already was reeling from a devastating earthquake in January 2010 that killed more than 300,000.

In a study titled "Population genetics of Vibrio Cholerae from Nepal: An identical clone in Nepal and the Haitian outbreak," researchers confirm the source of the cholera, and suggest how to prevent future outbreaks when international aid is rushed to disaster areas.

The study appears Tuesday August 23rd in mBio, a new online-only, open-access journal published by the American Society of Microbiology in partnership with the American Academy of Microbiology.

"The great similarity of Haitian cholera with Nepalese cholera is based upon the highest resolution DNA methods available, and point to a probable source of this devastating disease outbreak," said Dr. Paul Keim, Director of the TGen Pathogen Genomics Division and senior molecular biologist on the study. Dr. Keim assisted the FBI in tracking down the source of the 2001 anthrax letters case, which killed five people. He said similar genetic tracking techniques were used in investigating the Haitian cholera outbreak.

According to Dr. Keim, methods pioneered during the anthrax letter forensic investigation and today's greatly diminished costs for whole genome sequencing make it possible to apply this powerful technology to new and critical public health challenges. Dr. Keim is a Regents Professor at Northern Arizona University (NAU), whose Center for Microbial Genetics and Genomics also contributed to the study.

Dr. Keim praised TGen's collaborators at the National Public Health Laboratory in Nepal, and at the National Food Institute in Denmark, where the study's senior author, Dr. Frank M. Aarestrup, is head of the Antimicrobial Resistance and Molecular Epidemiology Unit.

"This study highlights how rapidly infectious diseases might be transmitted globally through international travel, and how public health officials need to use advanced molecular tools, along with standard epidemiological analyses, to quickly and accurately determine sources of outbreaks," said Dr. Aarestrup, who also is head of both the World Health Organization's Collaborating Centre for Antimicrobial Resistance among Foodborne Pathogens and of the European Union (EU) Reference Laboratory for Antimicrobial Resistance.

Dr. Lance Price, an associate professor at TGen and co-author of the new study, said the investigation into the source of Haitian cholera could help prevent such outbreaks in the future.

"This effort validates the power of advanced molecular tools in investigating outbreaks of this nature," Dr. Price said. "The goal now should be finding ways to prevent such outbreaks, perhaps through screening prior to deployment. This study is not about placing blame, it's about preventing such disasters in the future."

Researchers confirmed the source of the outbreak by comparing the DNA of 24 cholera samples (the bacterium Vibrio cholera) from five different districts in Nepal with 10 samples of cholera from Haiti. All 24 samples from Nepal matched the samples from Haiti. Some of the samples, the report said, "were almost identical."

Dr. Price said the TGen findings makes a very strong case for the source of the cholera, and aligns with a recent report by the U.S. Centers for Disease Control and Prevention, which said "evidence strongly suggests" that the Nepalese soldiers were the source of the outbreak. The CDC study was based on the fact that the first cholera infections in Haiti were downstream from the Nepalese, and occurred shortly after the soldiers' arrival.

"Our studies are complementary and together make a definitive case for a Nepalese origin to the outbreak," Dr. Price said.

###

About TGen

The Translational Genomics Research Institute (TGen) is a Phoenix, Arizona-based non-profit organization dedicated to conducting groundbreaking research with life changing results. Research at TGen is focused on helping patients with diseases such as cancer, neurological disorders and diabetes. TGen is on the cutting edge of translational research where investigators are able to unravel the genetic components of common and complex diseases. Working with collaborators in the scientific and medical communities, TGen believes it can make a substantial contribution to the efficiency and effectiveness of the translational process. TGen is affiliated with the Van Andel Research Institute in Grand Rapids, Michigan. For more information, visit: www.tgen.org.


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TGen and DTU researchers track source of Haitian cholera outbreak [ Back to EurekAlert! ] Public release date: 23-Aug-2011
[ | E-mail | Share Share ]

Contact: Steve Yozwiak
syozwiak@tgen.org
602-343-8704
The Translational Genomics Research Institute

Lessons from 2001 anthrax case help pinpoint source of disease

FLAGSTAFF, Ariz. Aug. 23, 2011 Employing technology that reads the entire DNA code, researchers led by the Translational Genomics Research Institute (TGen) and the Technical University of Denmark (DTU) have pinpointed the source of a cholera outbreak in Haiti that killed more than 6,000 people and sickened 300,000.

Using whole genome sequencing, which spells out the billions of chemical bases in DNA, TGen and DTU provided the strongest evidence yet that peacekeepers from Nepal, where cholera is widespread, brought the disease to Haiti, which already was reeling from a devastating earthquake in January 2010 that killed more than 300,000.

In a study titled "Population genetics of Vibrio Cholerae from Nepal: An identical clone in Nepal and the Haitian outbreak," researchers confirm the source of the cholera, and suggest how to prevent future outbreaks when international aid is rushed to disaster areas.

The study appears Tuesday August 23rd in mBio, a new online-only, open-access journal published by the American Society of Microbiology in partnership with the American Academy of Microbiology.

"The great similarity of Haitian cholera with Nepalese cholera is based upon the highest resolution DNA methods available, and point to a probable source of this devastating disease outbreak," said Dr. Paul Keim, Director of the TGen Pathogen Genomics Division and senior molecular biologist on the study. Dr. Keim assisted the FBI in tracking down the source of the 2001 anthrax letters case, which killed five people. He said similar genetic tracking techniques were used in investigating the Haitian cholera outbreak.

According to Dr. Keim, methods pioneered during the anthrax letter forensic investigation and today's greatly diminished costs for whole genome sequencing make it possible to apply this powerful technology to new and critical public health challenges. Dr. Keim is a Regents Professor at Northern Arizona University (NAU), whose Center for Microbial Genetics and Genomics also contributed to the study.

Dr. Keim praised TGen's collaborators at the National Public Health Laboratory in Nepal, and at the National Food Institute in Denmark, where the study's senior author, Dr. Frank M. Aarestrup, is head of the Antimicrobial Resistance and Molecular Epidemiology Unit.

"This study highlights how rapidly infectious diseases might be transmitted globally through international travel, and how public health officials need to use advanced molecular tools, along with standard epidemiological analyses, to quickly and accurately determine sources of outbreaks," said Dr. Aarestrup, who also is head of both the World Health Organization's Collaborating Centre for Antimicrobial Resistance among Foodborne Pathogens and of the European Union (EU) Reference Laboratory for Antimicrobial Resistance.

Dr. Lance Price, an associate professor at TGen and co-author of the new study, said the investigation into the source of Haitian cholera could help prevent such outbreaks in the future.

"This effort validates the power of advanced molecular tools in investigating outbreaks of this nature," Dr. Price said. "The goal now should be finding ways to prevent such outbreaks, perhaps through screening prior to deployment. This study is not about placing blame, it's about preventing such disasters in the future."

Researchers confirmed the source of the outbreak by comparing the DNA of 24 cholera samples (the bacterium Vibrio cholera) from five different districts in Nepal with 10 samples of cholera from Haiti. All 24 samples from Nepal matched the samples from Haiti. Some of the samples, the report said, "were almost identical."

Dr. Price said the TGen findings makes a very strong case for the source of the cholera, and aligns with a recent report by the U.S. Centers for Disease Control and Prevention, which said "evidence strongly suggests" that the Nepalese soldiers were the source of the outbreak. The CDC study was based on the fact that the first cholera infections in Haiti were downstream from the Nepalese, and occurred shortly after the soldiers' arrival.

"Our studies are complementary and together make a definitive case for a Nepalese origin to the outbreak," Dr. Price said.

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About TGen

The Translational Genomics Research Institute (TGen) is a Phoenix, Arizona-based non-profit organization dedicated to conducting groundbreaking research with life changing results. Research at TGen is focused on helping patients with diseases such as cancer, neurological disorders and diabetes. TGen is on the cutting edge of translational research where investigators are able to unravel the genetic components of common and complex diseases. Working with collaborators in the scientific and medical communities, TGen believes it can make a substantial contribution to the efficiency and effectiveness of the translational process. TGen is affiliated with the Van Andel Research Institute in Grand Rapids, Michigan. For more information, visit: www.tgen.org.


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Source: http://www.eurekalert.org/pub_releases/2011-08/ttgr-tad082211.php

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